Decreased synthesis of ribosomal proteins in tauopathy revealed by non‐canonical amino acid labelling
نویسندگان
چکیده
منابع مشابه
Identification of secreted bacterial proteins by noncanonical amino acid tagging.
Pathogenic microbes have evolved complex secretion systems to deliver virulence factors into host cells. Identification of these factors is critical for understanding the infection process. We report a powerful and versatile approach to the selective labeling and identification of secreted pathogen proteins. Selective labeling of microbial proteins is accomplished via translational incorporatio...
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Normal human lymphoblasts starved for each of several essential, but not nonessential, amino acids had decreased DNA and RNA synthesis but no change in free intracellular purine nucleotides. The rates of purine nucleotide synthesis via the de novo and salvage pathways were measured by incorporating [14C]formate and [“C]hypoxanthine labels, respectively, into lymphoblasts starved for an amino ac...
متن کاملA comparative study of ribosomal proteins: linkage between amino acid distribution and ribosomal assembly
BACKGROUND Assembly of the ribosome from its protein and RNA constituents must occur quickly and efficiently in order to synthesize the proteins necessary for all cellular activity. Since the early 1960's, certain characteristics of possible assembly pathways have been elucidated, yet the mechanisms that govern the precise recognition events remain unclear.We utilize a comparative analysis to i...
متن کاملSelective identification of newly synthesized proteins in mammalian cells using bioorthogonal noncanonical amino acid tagging (BONCAT).
In both normal and pathological states, cells respond rapidly to environmental cues by synthesizing new proteins. The selective identification of a newly synthesized proteome has been hindered by the basic fact that all proteins, new and old, share the same pool of amino acids and thus are chemically indistinguishable. We describe here a technology, based on the cotranslational introduction of ...
متن کاملDecreased expression of ribosomal proteins in human age-related cataract.
PURPOSE To identify lens epithelial genes with altered expression levels in age-related human cataract. METHODS Epithelia from age-related cataracts and from normal lenses were microdissected and RNA was extracted. RNAs were compared for gene expression differences by RT-PCR differential display. Transcripts exhibiting altered levels of gene expression were cloned and identified by sequencing...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 2019
ISSN: 0261-4189,1460-2075
DOI: 10.15252/embj.2018101174